Ensure data accuracy of your reader. Read-out was performed within 30 minutes with a microtiter plate reader at 450 nm (Versamax, Molecular Devices). Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. The absorbance at wavelength 450 nm was detected using a micro-plate reader (VersaMax, Molecular Devices, USA). FAST AND ACCURATE METERTECH Microplate Reader $3,650. g. The Versamax Microplate Reader is built around a grating monochromator. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. 5577; General Inquiry Form; Mon-Fri 8:00am - 5:00pmSpectraMax and VersaMax . +06-6399-8211 Fax +06-6399-8212 ©2002 Molecular Devices Corporation. Unit price / per . The plate was read at 570 nm using an ELISA reader (VERSAMax, Molecular Devices, VWR, Philadelphia, PA, USA). from Molecular Devices Corporation. Model: VERSAmax Tunable Microplate Reader. for cell lysis via visual inspection and optical microscopy (VersaMax, Molecular Devices, Sunnyvale, CA, USA) observation and checked by measuring the absorbance at OD680. Finally, a 5% glucose solution was added to the suspension of CPN and FCPN as a freeze-dried protective agent. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. 6 nm, respectively, by ELISA microplate reader (VERSAmax, Molecular Devices, USA). The optical density was measured at a wavelength of 450 nm using VersaMax (Molecular Devices, Seoul, South Korea). 2. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. and California VERSAMAX Tunable Microplate Reader 100-240V - 4A 50-60 HZ T4. Whether its cell line development, 3D biology, drug screening—or a combination of the three—our technology maximizes throughput and reproducibility to give. HUVECs were seeded into 96-well plates at a density of 30,000 cells/well and allowed to attach for 12 h. SpectraMax Plus384, 190, 340PC384, and VersaMax Installation Guide Author: Molecular Devices Subject: Installation Guide Keywords: SpectraMax Versa and others Detection Platform Created Date: 5/17/2023 2:14:27 PM At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. All parts of the SpectraTest ABS1 Validation Plate are warranted for a period of ninety (90) days from the original date of delivery. If the computer is connected to the Internet: Select Search Automatically for updated driver software. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein. Cells were treated with 150 μl of dimethylsulfoxide (DMSO) and absorbance was quantified at 540 nm using the microplate spectrophotometer system (VersaMax, Molecular Devices, Sunnyvale, CA, USA). It also integrates with the. 1. Finally, 0. This technical note is designed to provide information . TECHNICAL NOTE. Then the absorbance at 570 nm was measured using a microplate reader (VersaMax, Molecular Devices, San Jose, CA, USA). 23,225, Thermo Fisher Scientific). While the cell covers about. 00. The collaborative space brings customers and researchers into the lab to test automated workflows for organoid culturing and screening, with guidance from in-house. The well plates were then washed with 1% acetic acid and dried. Cell viability was assessed using the WST-1 assay. Table of Contents. 0A Sunnyvale, CA Made in USA Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). 00 Sale price $ 0. 00 Sale price $ 0. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Color intensities were measured using microplate reader (Versamax, Molecular Devices, Inc. Molecular Devices VersaMax Tunable Temperature range between 4°C above ambient to 45°C Digital controller and display Sold as-is The Molecular Devices VersaMax. 0A-Sunnyvale, CA-Made in USASpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. The absorbance was measured at 490 nm using a VersaMax ELISA reader (Molecular Devices, Sunnyvale, California, United States). The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. VersaMax™ Tunable Microplate Reader, Molecular Devices. 4. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. Absorbance was read at 412 nm every minute for 10 min using a VersaMax (Molecular Devices, San Jose, CA) spectrophotometer. Four wash/rinse bottles are included and configurable for both 96- and 384. HL-60 cells were plated at a density of 3×105 cells/well on 6-well plates. Manufacturer: Molecular Devices Model: VERSA MAX Turnable Microplate reader Further Information Descriptions, data, dimensions and quantities are based on the subjective beliefs of the auctioneer and are created to the best of our knowledge and belief. 00. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Find Fanuc, Molecular Devices, Eagle, and GE for sale on Machinio. Home - University of Victoriaimported directly into Excel (Microsoft, Redmond, WA) from the ELISA microplate reader (VersaMax; Molecular Devices, Sunnyvale, CA, USA). The plate was read at 570 nm using an ELISA reader (VERSAMax, Molecular Devices, VWR, Philadelphia, PA, USA). Briefly, 100 μl of cell culture medium was mixed with 100 μl of Griess reagent [equal volumes of 1% (w/v) sulfanilamide in 5% (v/v) phosphoric acid and 0. Read-out was performed within 30 minutes with a microtiter plate reader at 450 nm (Versamax, Molecular Devices). Although this manual has been prepared with every precaution to ensure accuracy, Molecular DevicesThe amount of extracted crystal violet was measured at 595 nm using a microplate reader (VersaMax, Molecular Devices Co. VERSAMAX. The SpectraMax Plus 384 Microplate Spectrophotometer is a versatile and reliable instrument that can measure absorbance in the UV and visible range. The robot-compatible VersaMax Reader is cost-effective—users never have to buy filters. Measurement of changes in BDNF production and release was done byMicroplate Readers. ). Molecular Devices is one of the leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical and biotherapeutic development. The mouse hippocampal neuronal cell line HT22 is frequently used as an in vitro model to investigate the role of hippocampal cholinergic neurons in cognitive functions. 0200-6117. Cell viability was represented by percentage values compared to a control and then used to calculate the number of viable cells. FAST AND ACCURATE METERTECH Microplate Reader $3,650. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. versamax, vmax, emax, uvmax, flexstation, softmax pro 5 gxp software, spectratest, molecular devices automation partners, gemini, lmax, assay protocols, data display, data analysis, assay. To update the driver. The absorbance was measured using a microplate reader (VERSAmax; Molecular Devices, San Jose, CA, USA) at an excitation wavelength of 530–560 nm. Although this manual has been prepared with every precaution to ensure accuracy, Molecular DevicesSpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. VersaMax Absorbance Microplate Reader memungkinkan pemilihan hingga dua panjang gelombang sekaligus untuk deteksi absorbansi dalam. The Molecular Devices VersaMax Microplate Reader is a high-performance laboratory instrument designed for fast and accurate measurements of absorbance, fluorescence, and luminescence from 6 to 384-well microplates. The in vitro differentiation of BMDMs. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. 1 mL. +81-6-6399-8211 fax +81-6-6399-8212 Nihon Molecular Devices Tokyo Tel. Molecular Devices Corporation. Molecular Devices versamax Operator's Manual (47 pages) Tunable Microplate Reader. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. Florescence intensity was monitored on a Gemini XPS fluorescence microplate reader (Molecular Devices, Sunnyvale, CA, USA) set for 488 nm excitation and 520 nm emission. . Invasion assayCells were seeded into the top chamber of a 96-well matrigel-coated plate with 8-m-pore polyethylene terephthalate membrane inserts (Corning). The Molecular Devices VersaMax Microplate Reader is a high-performance laboratory instrument designed for fast and accurate measurements of absorbance, fluorescence, and luminescence from 6 to 384-well microplates. Microplate readers from Molecular Devices are designed to provide consistent performance over a long period of time. Analytical Technologies Group specializes in the maintenance and repair of Molecular Devices microplate readers, including the SpectraMax® and VersaMax™ absorbance readers. S. After incubation, the absorbance of the plate was measured at 450 nm using a microplate reader (VERSAmax™, Molecular Devices Corp. Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. The value for non-specific absorption at 600 nm was subtracted. The assay procedure was in accordance with the manufacturer's instructions, and the results were analyzed with a microplate reader (VersaMax; Molecular Devices Ltd. 2. For more sample throughput, the system can be easily integrated into a full robotic The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. SoftMax® Pro, the industry-leading microplate reader software, is included at no additional charge. All the experiments were conducted at. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. For more information, please visit us at 160 µL of sample solutions/positive control (ascorbic acid) were mixed with 40 µL of DPPH solution and incubated at room temperature for 30 min. For quantitative analysis, the stains were re-dissolved in isopropanol, and the absorbances were measured at 520 nm using a spectrophotometer (VersaMax, Molecular Devices, CA, USA). Technical Specifications. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The activity was measured at 40 °C following o-nitrophenol (ONP) release at 405 nm using a microplate reader (Versamax, Molecular Devices, San Jose, CA, USA). All parts of the SpectraTest ABS1 Validation Plate are warranted for a period of ninety (90) days from the original date of. A. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. This manual is subject to change without notice. Molecular devices The VersaMax Absorbance Microplate Reader costs between $5,500 and $6,600. [Google. 003 OD ±1. Cell viability was calculated using the following formula, where OD t is the absorbance of the treated cells and OD c is the absorbance of control cells: Cell viability = O D t / O D c. SpectraTest® Validation Package. $1,195. All analyses were performed three times, and the mean values were used for further calculations. Indocyanine Green Depuration Assay. Used. Microwell plates, black (96-well) Rocking table with adjustable lifting frequency. Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the VERSAmax Tunable Microplate Reader are warranted for a period of one (1) year from the original date of delivery. The small footprint and touchscreen control allows for easy laboratory setup with minimal investment of time, cost, and effort. Absorbance was read on a spectrophotometer (VersaMax, Molecular Devices) with SoftMax Pro GxP v5 software. With its advanced features and user-friendly interface, the VersaMax is the perfect choice for a wide range of research. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Plates were read using a microplate reader (VersaMax, Molecular Devices) at 595 nm. measured over 60min using a microplate reader (VersaMax, Molecular Devices, Sunnyvale, CA) at a wavelength of 405nm. , Sunnyvale, CA, USA). All cell lines were cultivated for 24 h in 96-well cell culture plates with an initial concentration of 1×10 5 cells/mL. The system provides automation for up to 50 For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. The optical densities (ODs) of the reactants were measured at 450 nm using a microplate spectrophotometer (Versamax, Molecular Devices, CA, USA). 1 N NaOH (50 μl) followed by absorbance measurement at 405 nm with a plate reader (VersaMax, Molecular Devices, Sunnyvale, CA). We would like to show you a description here but the site won’t allow us. High-Throughput Screening. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers begining with. Then, the cultured cells were treated with different. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. The sections were stained with hematoxylin and eosin (H&E) and. After treatment with GA3The absorbance was measured with a microplate reader (VERSAmax, Molecular devices, San Jose, CA, USA) at 570 nm according to the manufacturer’s instructions. Lipid accumulation was assessed by ORO staining. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747. Fabrication of Microfiber-Based Strain SensorsWe would like to show you a description here but the site won’t allow us. VERSA MAX-LUNABLE, MOLECULAR DEVICES, MICROPLATE R. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. New and used Molecular Devices VersaMax's for sale on LabX. It also integrates with the powerful SoftMax Pro Software for. The absorbance was assumed to be 100% for the control group. Bacterial growth was determined turbimetrically using the microplate ELISA reader VERSAmax (Molecular Devices). Molecular Devices Corporation SpectraTest ABS1 Validation Plate Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1. harveyi BB152 was used as the AI-2-producing positive control (prepared as reported (11)). EUROPE. These models can no longer be repaired. Molecular Devices SoftMax Pro 5. Oil red O staining. Activity was calculated as picomoles of substrate hydrolyzed per min. SoftMax® Pro 5 Software from Molecular Devices offers extensive data capture, analysis, and reduction capabilities for its absorbance, luminescence,. Versamax Microplate Reader dibangun di sekitar monokromator kisi. 8), 40 μL of tyrosinase, and 40 μL of L-DOPA were put in each well. Monday to Friday 8am - 5pm GMT. This robust model allows precise wavelength detection in. Spectranet customer portalThe absorbance was recorded at 532 nm using a microplate reader (VERSAmax, Molecular Devices, Sunnyvale, USA). Biosystems CytoFluor 4000 Multi Well Plate Reader $ 1,995. Finally, a 5% glucose solution was added to the suspension of CPN and FCPN as a freeze-dried protective agent. Select Add or Remove Programs system settings. The percentage of cytotoxicity was calculated using the following formula: cytotoxicity (%) = [1 − (At/As) × 100], where At and As refer to the absorbance of a tested. The quantity of nitrite in the samples was calculated using the concentration of sodium nitrite as a standard. Ann Arbor, MI, USA). The. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. (VersaMax, Molecular Devices, Sunnyvale, CA, US). This. The VersaMax measures thMolecular Devices memberdayakan para ilmuwan untuk memajukan diagnosis dini dan terapi yang lebih aman untuk pasien. Accession codes. HT22 cells are derived from hippocampal neuronal HT4 cells. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. The absorbance at 570 nm was determined by VersaMax (Molecular Devices, CA) to estimate MTT-formazan production after 24, 48, and 72 hours of incubation to evaluate the proliferation rate. After washing in PBS at least 4 times to remove all extracellular dye, samples were fixed using 4 % PFA/PBS for. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Filterless. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. For pinocytosis, cells in wells were washed in PBS and 5 mg/ml of HPTS dye (in PBS) added and incubated with gentle rocking at 30 °C for 120 min. StakMax. Turn the reader on and secure the USB cable. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. The narrowly focused light beam and optical design give you the same high performance with round bottom, flat bottom, or half area well plates. spectrophotometer (VERSAmax, Molecular Devices). A. reader (VersaMax, Molecular devices, San Jose, CA, USA). 5 × 10 6 cells per well inoculated into 6-well plates in culture medium with CSF1 (10 ng/mL) for 7. GABA concentrations in samples were calculated from calibration curves using the standard solutions. Read-out was performed within 30 minutes with a microtiter plate reader at 450 nm (Versamax, Molecular Devices). S. 3. New and used Molecular Devices VersaMax's for sale on LabX. 635. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. At All-usermanuals. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. 3 Methods 1. #VERSAMAX) SoftMax Pro GxP Software version 5. We would like to show you a description here but the site won’t allow us. No accessories are needed and SoftMax® Pro Software. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. ,, [28]. And the VERSAmax reader is robotics-compatible. The signal was developed using 3,3′, 5,5′-tetramethylbenzidine after streptavidin–peroxidase treatment. All. Preparation of cytoplasmic and nuclear protein. Absorbance was read on a spectrophotometer (VersaMax, Molecular Devices) with SoftMax Pro GxP v5 software. BMG Labtech - THERMOstar. We would like to show you a description here but the site won’t allow us. This document is provided to customers who have purchased Molecular Devices, Inc. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340–850nm). The cortisol-driven molecular programming of HPI axis development has focused only on GR signalling in fishes 12,13,14,15,16,17. The absorbance was detected at 570 nm with a Microplate Reader (VersaMax, Molecular Devices, Sunnyvale, CA). 00 Regular price. SpectraMax®, VersaMax, VMax. The plates were incubated at RT for an additional 25 min to stabilize the luminescence signals followed by the measurement of luminescence using a microplate reader (VersaMax, Molecular Devices, CA, USA) (n = 6). molecular devices | 1311 orleans drive | sunnyvale, cA 94089 usA | email: info@moldev. Enzyme activity was. Our products enable scientists to improve productivity and efficiency in scientific discovery and research. The. The Molecular Devices VersaMax Absorbance Microplate Reader enables both precise and accurate detection at wavelengths in the visible spectrum. End-point IgG titers were established as the mean plus three standard deviations of. 8. 747. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0140 Rev. ® ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. A solution of 10 millimolar L-DOPA (40 µL) was added to each sample and the blank. pneumoniae ATCC49619 was used as reference control strain as recommended (Clinical and Laboratory Standards Institute. Wavelength Accuracy. g. The rate of cell viability was calculated using the following formula:The conversion of NADP+ to NADPH was measured as absorbance (340 nm) on a microplate reader (VersaMax, Molecular Devices Co. In this study, we have prepared thermoreversible PG containing emodin with high emodin solubility and the gelation temperature suited to physiological use. Molecular Devices versamax Operator's Manual (47 pages) Tunable Microplate Reader. 03 billion doses of. The eight-channel system- consisting of 8 sample beams and 8 reference beams delivers both. SpectraMax® i3 Multi-Mode Detection Platform. Briefly, MTT (200 μl, 2 mg/ml) was added to the wells and incubated for 4 h at 37°C. 0 OD Kinetic Read Time: 9 Second Minimum Interval Temperature Uniformity ±0. Molecular Devices公司的VersaMax读 板机为光栅型单色器读板机，拥有灵活检 测优势，无需加配额外的滤光片。 可调波长，无需滤光片，灵活 VersaMax读板机使用光栅单色器来选择 波长，其结果是选择确切的检测波长应用 于每个实验。波长范围从340nm到 With the VersaMax microplate reader, you will never have to buy another filter. 00 Read more Molecular Devices Tunable Microplate Reader VersaMaxMicroplate reader with 450 nm filter, such as VersaMax (Molecular Devices, Sunnyvale, CA). An ELISA plate reader (VERSAmax; Molecular Devices, LLC, Sunnyvale, CA, USA) was used to measure the absorbance at 480 nm. ¨Molecular Devices is a life-science technology innovator Our integrated hardware and software solutions enable automated workflows—from start to finish—for any research application. Molecular Devices VersaMax Tunable Microplate Reader. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. Optical Densitiy (OD) values were processed with a data reduction software (SoftMax Pro, Molecular Devices) using a 4-parameter logistic fitting algorithm to the standard curve, allowing the determination of antibody. 0. 3 Methods 1. Tristar 3 Multimode Microplate Reader. 2. The primers for the human genes GAPDH, SOD1, SOD2, CAT, GPX1, CCS, PRDX6, FOXO3, CDKN2A,. The optical density (OD) was measured at 570 nm (with a reference at 690 nm) by UV-visible spectrophotometer (VersaMax, Molecular Devices, CA, USA) in triplicates. After waiting 15 minutes for colour development at room temperature, the reaction was stopped with 1 M H 2 SO 4, and optical density values were read using an ELISA reader (Versamax, Molecular Devices, LLC, USA) at a wavelength of 492 nm, and the results were given as OD values. 0 (Molecular Devices, Sunnyvale, CA). To test if sodium arsenite and dimethylarsenic acid could stimulate apoptosis in 3 cells, oc cell cycle redistribution was studied using flow cytometry with propidium iodide staining. Acetylcholinesterase activity was calculated as the rate of change of absorbance per minute during the linear portion of the reaction and normalized by protein concentration as determined by a Coomassie. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. For the MTS assay, the CellTiter 96® AQ ueous One Solution Cell Proliferation Assay kit was used following the manufacturer's instruction. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. We would like to show you a description here but the site won’t allow us. 9 in. SpectraMax and VersaMax Plate Readers Operator s Manual -- 0112-0126 Rev A Molecular De. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Flexible calculations Data analysis features are available such as 21 different curve fit options, simplified cross-plate analysis and interpolation, and automatic calculations. The amount of MTT reduction was measured immediately by detecting absorbance in a microplate reader (Versamax, Molecular Devices) at a wavelength of 570 nm and a reference wavelength. The objectives of this study were to determine phytochemical compositions, chemiluminescence antioxidant activities, and neuroprotective effects on PC12 cells for water, methanol, and 95% ethanol extracts of the air-dried fruit of <i>Terminalia chebula</i> Retzius. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Page 6 SpectraMax Plus384, 190, 340PC384, and VersaMax. Tristar 3 Multimode Microplate Reader. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. HT22 cells are derived from hippocampal neuronal HT4 cells. With a wavelength. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with streamlined workflows to ensure data integrity. The optical density (OD) of the mixture was measured at 490 nm with a microplate absorbance reader (VersaMax (Molecular Devices), Shanghai, China). 0 out of 5 $ 2,999-20%. Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 5 mM was used to make the. Then the medium was removed and 200 μL of DMSO was added to each well to solubilize the formazan crystals. 0 N sulfuric acid. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340–850nm). StakMax Microplate Handling Stacker. Model : VersaMax . Software (optional), such as Softmax Pro (Molecular Devices). The wells were visualized using o-phenylenediamine (OPD), and the absorbance was read with a multiwall spectrophotometer (VERSAmax, Molecular Devices, Sunnyvale, CA, USA) at 490 nm. 8). 3860 N First Street San Jose,CA 95134 USA +1 408. Spectranet customer portal. Afterward, HUVECs were incubated with EPC-EVs, TFEB-EVs, or PBS for 24 h or 48 h. The samples were incubated with FBS, and the optical density of particles was determined with a microplate reader (VERSAmax, Molecular Devices, Sunnyvale, CA) by measuring the absorbance at 560 nm for 2 h. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. 파장 정확도 : < ±0. 4 (Molecular Devices cat. , Sunnyvale, USA). Wavelength Selection. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. 专为科研检测平台量身打造的灵活且强大的小型微孔板读板机（酶标仪），具备终点法和动力学检测模式，标准配置6块滤光片，覆盖蛋白定量、细胞活力、农药残留、ELISA实验等. SoftMax Pro 7. Molecular Devices Versamax; Molecular Devices Vmax; Molecular Devices Fmax Fluorescence; Packard Fluorocount Fluorescence; Packard Spectracount; Tecan Spectrafluor Plus Multilabel; Perkin Elmer HTS-7000 Plus Multilabel; Skatron Skanstacker 300 Washer; Berthold LB96P Luminescence;Finally, cells were incubated with 1% SDS for 2 h at RT to release the dye from the cells, and the cell proliferation rate was assessed by quantification of the amount of dye released from the cells using a spectrophotometer (VersaMax, Molecular Devices) at the wavelength of 570 nm. We would like to show you a description here but the site won’t allow us. 99 shipping. The absorbance was detected at 517 nm wavelength using a microplate reader (VersaMax, Molecular devices, USA). 0 out of 5 $ 2,999. The BMDM cells were plated into a 6-well culture plate at a density of 2. Fluorescence-activated cell-sorting analysis For analysis of DC surface molecules, 5×10 5 DCs were treated with soluble OVA (20 μg/mL), PLGA NPs (20 μg/mL OVA), PEI-coated PLGA NPs (20 μg/mL OVA), or 1 μg/mL. recombinant proteins, including monoclonal antibodies. For determination of the optimal temperature, EfmTDC was incubated in 100 mM sodium acetate buffer (pH 5. StakMax Microplate Handling System. VERSAmaxTM Tunable Microplate Reader Operator s Manual for Instruments with Serial Numbers Beginning. That's why our team is at your disposal the majority of time. , USA) at 490 nm. from Molecular Devices Corporation. ces at one of the following telephone numbers: 408 747-1700 800 635-5577 U. Ensure data accuracy of your reader. The absorbance was measured at 570 nm using a microplate reader (VersaMax, Molecular Devices). SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. 5°C @ 37°C Well-to. One microgram of RNA was. 72 MB. Get pricing and purchase lab equipment and instrumentation today. 0, 0. After addition of stop solution, the optical density of samples were read at wavelengths of 450 and 570 nm (Versamax, Molecular Devices Co. Sponsored. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. s. The. Furnace Manuals. StakMax. Table 1. Manual for Molecular Devices versamax Laboratory Equipment (47 pages) Specifications: 1379/1379020-versamax. MiR-21. JEG-3 cells were transfected with myc-tagged NDRG1 wild type or deletion mutants at the N- or C- terminal as depicted in Figure 5A. SpectraMax Plus384 SpectraMax 190 SpectraMax 340PC384 VersaMax Microplate Spectrophotometer Operator s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Used. TECHNICAL NOTE. The VersaMaxTM Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. 00 USD. DMEM medium supplemented with the same volume of kit reagent on an empty well was used as a blank. Cell viability was represented by percentage values compared to a control and then used to calculate the number of viable cells. 5 mg/mL) in the range of 5–100 μg per 0. Negative (phosphate buffered saline) (Gibco Waltham, MA, USA) and positive controls. Supplier: Molecular Devices. Turn the reader on and secure the USB cable. Molecular Devices, LLC. Use Windows Search to search for “Add or Remove”. This reliable model allows for. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. This. After 72-h of incubation, CellTiter 96 Aqueous One Solution was added and cells were incubated for a further 2 h. After freeze-drying, the.